Name: trpv4 egfp
Brand: Mutant Mouse Resource & Research Center
Rating: 90 (1 reviews)

trpv4 egfp (Mutant Mouse Resource & Research Center)

Mutant Mouse Resource & Research Center trpv4 egfp

<t>TRPV4</t> expression is elevated in skin biopsies of CIP patients and TRPV4 function is required for generating mouse models of both allergic and non-allergic chronic itch. A, The bar charts show the averaged itch numerical rating scale (NRS) scores and mRNA transcripts for TRPV4 and TRPV3 in CIP patients and health controls.. *p<0.05, **p<0.01, Student’s t test; n=8. n.s. not significant versus control group. B, Spontaneous scratches in Trpv4+/+ and Trpv4−/− mice after 7 days of AEW treatment. ***p<0.001, Student’s t test; n=7.. C, Spontaneous scratches in Trpv4+/+ mice after 7 days of AEW treatment were reduced by HC067 (20 mg/kg, i.p or topical application) compared with vehicle. *p<0.05, Student’s t test; n=8–9. D, Spontaneous scratches after the treatment with the 1:1 mixture of acetone and ether followed by 0.9% NaCl, 0.45% NaCl, or distilled water, respectively. **p<0.01, ANOVA; n=5–7. E, Spontaneous scratches in Trpv4+/+ and Trpv4−/− mice after SADBE treatment. *p<0.05, Student’s t test; n=6. F, SADBE-induced spontaneous scratches in Trpv4+/+ mice after treatment with HC067 (20 mg/kg, i.p or topical application) or vehicle. *p<0.05, Student’s t test; n=5–6.

Trpv4 Egfp, supplied by Mutant Mouse Resource & Research Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trpv4 egfp - by Bioz Stars, 2026-02

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trpv4-egfp (human) in pegfp (Charite Research Organisation)

Charite Research Organisation trpv4-egfp (human) in pegfp

Trpv4 Egfp (Human) In Pegfp, supplied by Charite Research Organisation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trpv4-egfp (human) in pegfp/product/Charite Research Organisation
Average 90 stars, based on 1 article reviews

trpv4-egfp (human) in pegfp - by Bioz Stars, 2026-02

90/100 stars

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TRPV4 expression is elevated in skin biopsies of CIP patients and TRPV4 function is required for generating mouse models of both allergic and non-allergic chronic itch. A, The bar charts show the averaged itch numerical rating scale (NRS) scores and mRNA transcripts for TRPV4 and TRPV3 in CIP patients and health controls.. *p<0.05, **p<0.01, Student’s t test; n=8. n.s. not significant versus control group. B, Spontaneous scratches in Trpv4+/+ and Trpv4−/− mice after 7 days of AEW treatment. ***p<0.001, Student’s t test; n=7.. C, Spontaneous scratches in Trpv4+/+ mice after 7 days of AEW treatment were reduced by HC067 (20 mg/kg, i.p or topical application) compared with vehicle. *p<0.05, Student’s t test; n=8–9. D, Spontaneous scratches after the treatment with the 1:1 mixture of acetone and ether followed by 0.9% NaCl, 0.45% NaCl, or distilled water, respectively. **p<0.01, ANOVA; n=5–7. E, Spontaneous scratches in Trpv4+/+ and Trpv4−/− mice after SADBE treatment. *p<0.05, Student’s t test; n=6. F, SADBE-induced spontaneous scratches in Trpv4+/+ mice after treatment with HC067 (20 mg/kg, i.p or topical application) or vehicle. *p<0.05, Student’s t test; n=5–6.

Journal: The Journal of allergy and clinical immunology

Article Title: TRPV4-expressing Macrophages and Keratinocytes Contribute Differentially to Allergic and Non-allergic Chronic Itch

doi: 10.1016/j.jaci.2017.05.051

Figure Lengend Snippet: TRPV4 expression is elevated in skin biopsies of CIP patients and TRPV4 function is required for generating mouse models of both allergic and non-allergic chronic itch. A, The bar charts show the averaged itch numerical rating scale (NRS) scores and mRNA transcripts for TRPV4 and TRPV3 in CIP patients and health controls.. *p<0.05, **p<0.01, Student’s t test; n=8. n.s. not significant versus control group. B, Spontaneous scratches in Trpv4+/+ and Trpv4−/− mice after 7 days of AEW treatment. ***p<0.001, Student’s t test; n=7.. C, Spontaneous scratches in Trpv4+/+ mice after 7 days of AEW treatment were reduced by HC067 (20 mg/kg, i.p or topical application) compared with vehicle. *p<0.05, Student’s t test; n=8–9. D, Spontaneous scratches after the treatment with the 1:1 mixture of acetone and ether followed by 0.9% NaCl, 0.45% NaCl, or distilled water, respectively. **p<0.01, ANOVA; n=5–7. E, Spontaneous scratches in Trpv4+/+ and Trpv4−/− mice after SADBE treatment. *p<0.05, Student’s t test; n=6. F, SADBE-induced spontaneous scratches in Trpv4+/+ mice after treatment with HC067 (20 mg/kg, i.p or topical application) or vehicle. *p<0.05, Student’s t test; n=5–6.

Article Snippet: Adult male and female C57BL/6J mice (Jackson Laboratories), Trpv4 eGFP (Mutant Mouse Regional Resource Centers, MMRRC), Trpv4 − / − , 11 Kit W-sh/W-sh (Jackson Laboratories), Htr7 − / − (Jackson Laboratories), Htr2a − / − (a kind gift from Dr Jay Gingrich at the Columbia University) mice were used for the study.

Techniques: Expressing

TRPV4 is functionally expressed by mouse skin-resident cells. A, Double labeling reveals the co-localization of TRPV4-eGFP with K14 (left panel) and F4/80 (right panel) in skin sections from the Trpv4eGFP mice. Bar=50 μm. HF: hair follicle. Inset shows the magnification of boxed area. B, RT-PCR shows the correlation between TRPV4 and GFP in sorted GFP+ and GFP− ear skin single-cell suspensions. C, Flow cytometry analysis illustrates that TRPV4-eGFP is expressed in CD11b-positive skin-resident cells. D, Representative traces showing the GSK101-elicited large [Ca2+]i responses in freshly dissociated ear skin single-cell suspensions from Trpv4+/+ (left) but not Trpv4−/− (middle) mice. Arrows indicate the time points of GSK101 applications. Ionomycin (Ion) was used as positive control. Summarized data from in the right showing the percentages of GSK101-responsive skin cells isolated from the Trpv4+/+ and Trpv4−/− mice. ***p<0.001, Student’s t test; n=5–8. E, Time course (left) and representative current-voltage curves (middle) elicited by voltage ramps in the absence or presence of 0.3 μM GSK101 in the TRPV4-eGFP+ myeloid cells from the ear skin single-cell suspensions. HC067 at 5 μM markedly inhibited the GSK101-activated currents. Bar charts in the right illustrate summarized data. ***p<0.001, ANOVA; n=5.

Journal: The Journal of allergy and clinical immunology

Article Title: TRPV4-expressing Macrophages and Keratinocytes Contribute Differentially to Allergic and Non-allergic Chronic Itch

doi: 10.1016/j.jaci.2017.05.051

Figure Lengend Snippet: TRPV4 is functionally expressed by mouse skin-resident cells. A, Double labeling reveals the co-localization of TRPV4-eGFP with K14 (left panel) and F4/80 (right panel) in skin sections from the Trpv4eGFP mice. Bar=50 μm. HF: hair follicle. Inset shows the magnification of boxed area. B, RT-PCR shows the correlation between TRPV4 and GFP in sorted GFP+ and GFP− ear skin single-cell suspensions. C, Flow cytometry analysis illustrates that TRPV4-eGFP is expressed in CD11b-positive skin-resident cells. D, Representative traces showing the GSK101-elicited large [Ca2+]i responses in freshly dissociated ear skin single-cell suspensions from Trpv4+/+ (left) but not Trpv4−/− (middle) mice. Arrows indicate the time points of GSK101 applications. Ionomycin (Ion) was used as positive control. Summarized data from in the right showing the percentages of GSK101-responsive skin cells isolated from the Trpv4+/+ and Trpv4−/− mice. ***p<0.001, Student’s t test; n=5–8. E, Time course (left) and representative current-voltage curves (middle) elicited by voltage ramps in the absence or presence of 0.3 μM GSK101 in the TRPV4-eGFP+ myeloid cells from the ear skin single-cell suspensions. HC067 at 5 μM markedly inhibited the GSK101-activated currents. Bar charts in the right illustrate summarized data. ***p<0.001, ANOVA; n=5.

Techniques: Labeling, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, Positive Control, Isolation

TRPV4 channels expressed by macrophages and keratinocytes contribute differentially to allergic and non-allergic chronic itch. A, Representative images showing the TRPV4-eGFP+ cells in the skin of Trpv4eGFP mice treated with vehicle control and AEW. B, The epidermal thickness was significantly increased by AEW and SADBE treatments compared with their respective vehicle controls. ***p<0.001, Student’s t test; n=6–8. C, The number of TRPV4-eGFP+ dermal macrophages increased significantly following AEW or SADBE treatment. ***p<0.001, Student’s t-test; n=6–8. D, Relative TRPV4 mRNA in the skin of AEW- or SADBE-treated mice, *p<0.05, Student’s f test; n=6–8. E, Spontaneous scratching in the K14-Cre+ and K14-Cre− mice after AEW treatment, *p<0.05, Student’s f test; n=5. F, Spontaneous scratching in the Cx3cr1-Cre+ and Cx3cr1-Cre− mice after AEW treatment. Student’s t test; n=5. n.s. not significant. G, Spontaneous scratching in the K14-Cre+ and K14-Cre− mice after SADBE treatment. Student’s t test; n=8–9. n.s. not significant. H, Spontaneous scratching in the Cx3cr1-Cre+ and Cx3cr1-Cre− mice after SADBE treatment. **p<0.01, Student’s t test; n=9.

Journal: The Journal of allergy and clinical immunology

Article Title: TRPV4-expressing Macrophages and Keratinocytes Contribute Differentially to Allergic and Non-allergic Chronic Itch

doi: 10.1016/j.jaci.2017.05.051

Figure Lengend Snippet: TRPV4 channels expressed by macrophages and keratinocytes contribute differentially to allergic and non-allergic chronic itch. A, Representative images showing the TRPV4-eGFP+ cells in the skin of Trpv4eGFP mice treated with vehicle control and AEW. B, The epidermal thickness was significantly increased by AEW and SADBE treatments compared with their respective vehicle controls. ***p<0.001, Student’s t test; n=6–8. C, The number of TRPV4-eGFP+ dermal macrophages increased significantly following AEW or SADBE treatment. ***p<0.001, Student’s t-test; n=6–8. D, Relative TRPV4 mRNA in the skin of AEW- or SADBE-treated mice, *p<0.05, Student’s f test; n=6–8. E, Spontaneous scratching in the K14-Cre+ and K14-Cre− mice after AEW treatment, *p<0.05, Student’s f test; n=5. F, Spontaneous scratching in the Cx3cr1-Cre+ and Cx3cr1-Cre− mice after AEW treatment. Student’s t test; n=5. n.s. not significant. G, Spontaneous scratching in the K14-Cre+ and K14-Cre− mice after SADBE treatment. Student’s t test; n=8–9. n.s. not significant. H, Spontaneous scratching in the Cx3cr1-Cre+ and Cx3cr1-Cre− mice after SADBE treatment. **p<0.01, Student’s t test; n=9.

Techniques:

5-HT signaling is required for TRPV4-dependent chronic itch. A, Percentages of DRG neurons responding to skin superfusates from normal Trpv4+/+, AEW Trpv4+/+, and AEW Trpv4−/− mice. ***p<0.001, ANOVA. B, Percentages of 5-HT-responsive DRG neurons responding to the AEW skin superfusates from Trpv4+/+ and Trpv4−/− mice. ***p<0.001, Student’s t test. C, Schematic drawing of the 5-HT synthesis pathway. D and E, Spontaneous itching in AEW- (Fig 4, D) and SADBE- (Fig 4, E) treated mice with pCPA or pCPA+5-HTP. *p<0.05, ***p<0.001, ANOVA; n=8–10. F, Spontaneous scratching in the Htr2a+/+ and Htr2a−/− mice after SADBE treatment. *p<0.05, Student’s t test; n=5. G, Spontaneous scratching in mice treated with vehicle, Ketanserin, or Htr7 antagonist SB269970 (SB269) after SADBE treatment. *p<0.05, ANOVA; n=6. H, Spontaneous scratching in Htr7+/+ and Htr7−/− mice after SADBE treatment. Student’s t test; n=6. I, Spontaneous scratching in Htr2a+/+ and Htr2a−/− mice after AEW treatment. Student’s t test; n=9. J, Spontaneous scratching after AEW treatment in mice treated with vehicle, Htr7 antagonist SB269970 (SB269), or Ketanserin. **p<0.01, ANOVA; n=6–7. K, Spontaneous scratching after AEW treatment in the Htr7+/+ and Htr7−/− mice. ***p<0.001, Student’s t test; n=10–11. n.s. not significant versus vehicle group.

Journal: The Journal of allergy and clinical immunology

Article Title: TRPV4-expressing Macrophages and Keratinocytes Contribute Differentially to Allergic and Non-allergic Chronic Itch

doi: 10.1016/j.jaci.2017.05.051

Figure Lengend Snippet: 5-HT signaling is required for TRPV4-dependent chronic itch. A, Percentages of DRG neurons responding to skin superfusates from normal Trpv4+/+, AEW Trpv4+/+, and AEW Trpv4−/− mice. ***p<0.001, ANOVA. B, Percentages of 5-HT-responsive DRG neurons responding to the AEW skin superfusates from Trpv4+/+ and Trpv4−/− mice. ***p<0.001, Student’s t test. C, Schematic drawing of the 5-HT synthesis pathway. D and E, Spontaneous itching in AEW- (Fig 4, D) and SADBE- (Fig 4, E) treated mice with pCPA or pCPA+5-HTP. *p<0.05, ***p<0.001, ANOVA; n=8–10. F, Spontaneous scratching in the Htr2a+/+ and Htr2a−/− mice after SADBE treatment. *p<0.05, Student’s t test; n=5. G, Spontaneous scratching in mice treated with vehicle, Ketanserin, or Htr7 antagonist SB269970 (SB269) after SADBE treatment. *p<0.05, ANOVA; n=6. H, Spontaneous scratching in Htr7+/+ and Htr7−/− mice after SADBE treatment. Student’s t test; n=6. I, Spontaneous scratching in Htr2a+/+ and Htr2a−/− mice after AEW treatment. Student’s t test; n=9. J, Spontaneous scratching after AEW treatment in mice treated with vehicle, Htr7 antagonist SB269970 (SB269), or Ketanserin. **p<0.01, ANOVA; n=6–7. K, Spontaneous scratching after AEW treatment in the Htr7+/+ and Htr7−/− mice. ***p<0.001, Student’s t test; n=10–11. n.s. not significant versus vehicle group.

Techniques:

Platelets but not mast cells are required for TRPV4-dependent chronic itch. A, Immunofluorescent staining shows that TRPV4-eGFP was not colocalized with streptavidin, a mast cell marker. Bar=50 μm. B and C, Spontaneous scratching induced by AEW (Fig 5, B) or SADBE (Fig 5, C) treatment was not significantly affected in the sash mice. Student’s t test; n=6 −7. n.s. not significant versus wt control group. D, Platelet count in Pf4-Cre mice and Pf4-Cre+ mice 6 days after the DTX treatment. **p<0.01, Student’s t test; n=7–8. E and F, Spontaneous scratching responses induced by AEW (Fig 5, E) or SADBE (Fig 5, F) in the Pf4-Cre− mice (n=5 for AEW and SADBE) mice and the Pf4-Cre+ mice (n=6 for AEW and SADBE). *p<0.05, Student’s t test. G and H, Spontaneous scratching responses induced by AEW (Fig 5, G) or SADBE (Fig 5, H) in the absence or presence of eptifibatide or clopidogrel. *p<0.05, ANOVA; n=6.

Journal: The Journal of allergy and clinical immunology

Article Title: TRPV4-expressing Macrophages and Keratinocytes Contribute Differentially to Allergic and Non-allergic Chronic Itch

doi: 10.1016/j.jaci.2017.05.051

Figure Lengend Snippet: Platelets but not mast cells are required for TRPV4-dependent chronic itch. A, Immunofluorescent staining shows that TRPV4-eGFP was not colocalized with streptavidin, a mast cell marker. Bar=50 μm. B and C, Spontaneous scratching induced by AEW (Fig 5, B) or SADBE (Fig 5, C) treatment was not significantly affected in the sash mice. Student’s t test; n=6 −7. n.s. not significant versus wt control group. D, Platelet count in Pf4-Cre mice and Pf4-Cre+ mice 6 days after the DTX treatment. **p<0.01, Student’s t test; n=7–8. E and F, Spontaneous scratching responses induced by AEW (Fig 5, E) or SADBE (Fig 5, F) in the Pf4-Cre− mice (n=5 for AEW and SADBE) mice and the Pf4-Cre+ mice (n=6 for AEW and SADBE). *p<0.05, Student’s t test. G and H, Spontaneous scratching responses induced by AEW (Fig 5, G) or SADBE (Fig 5, H) in the absence or presence of eptifibatide or clopidogrel. *p<0.05, ANOVA; n=6.

Techniques: Staining, Marker

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